MERCK 100025.0500 Demi Fraser Broth With Antibiotics For Microbiology

MERCK 100025.0500 Demi Fraser Broth With Antibiotics For Microbiology (SD.M.100025.0500)

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MERCK 100025.0500 Demi Fraser Broth With Antibiotics For Microbiology

Description
Catalogue Number	100025
Overview	GranuCult™ is our new brand name for our existing granulated dehydrated culture media which apply to new EN ISO 11133:2014 and various other ISO standards and FDA-BAM / USDA-FSIS methods for testing of food and water samples. All these media are currently being updated to be fully compliant to the latest version of these standards and regulations with respect to composition and quality control and will be available shortly.

Description

Catalogue Number

100025

Overview

GranuCult™ is our new brand name for our existing granulated dehydrated culture media which apply to new EN ISO 11133:2014 and various other ISO standards and FDA-BAM / USDA-FSIS methods for testing of food and water samples. All these media are currently being updated to be fully compliant to the latest version of these standards and regulations with respect to composition and quality control and will be available shortly.

Product Information
HS Code	3821 00 00
Quality Level	MQ200

Physicochemical Information
pH value	7.0 - 7.4 (55 g/l, H₂O, 25 °C) (after autoclaving)
Solubility	55 g/l

Safety Information according to GHS
Storage class	10 - 13 Other liquids and solids
WGK	WGK 3 highly hazardous to water
Disposal	3 Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.

Storage and Shipping Information
Storage	Store at +15°C to +25°C.

Specifications
Appearance (clearness)	clear
Appearance (color)	yellowish-brown
pH-value (25 °C)	7.0 - 7.4
Growth promotion test in accordance with the current version of DIN EN ISO 11133. Mixed inoculum of Listeria, E.coli and E.faecalis Additionaly monoculture of E.coli and E.faecalis Afterwards confirmation on ALOA®-Medium resp. Tryptic Soy agar
Inoculum on reference medium (Listeria monocytogenes ATCC 13932 (WDCM 00021))	≤ 100
Inoculum on reference medium (Listeria monocytogenes ATCC 35152 (WDCM 00109))	≤ 100
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013))	≥ 1000
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012))	≥ 1000
Inoculum on reference medium (Enterococcus faecalis ATCC 19433 (WDCM 00009))	≥ 1000
Inoculum on reference medium (Enterococcus faecalis ATCC 29212 (WDCM 00087))	≥ 1000
Growth on ALOA® medium (Listeria monocytogenes ATCC 13932 (WDCM 00021))	> 10 colonies
Growth on ALOA® medium(Listeria monocytogenes ATCC 35152 (WDCM 00109))	> 10 colonies
Colonies (Listeria monocytogenes ATCC 13932 (WDCM 00021))	blue green colonies with opaque halo
Colonies(Listeria monocytogenes ATCC 35152 (WDCM 00109))	blue green colonies with opaque halo
Growth on Tryptic soy agar (Escherichia coli ATCC 25922 (WDCM 00013))	total inhibition
Growth on Tryptic soy agar (Escherichia coli ATCC 8739 (WDCM 00012))	total inhibition
Growth on Tryptic soy agar (Enterococcus faecalis ATCC 29212 (WDCM 00087))	< 100 colonies
Growth on Tryptic soy agar (Enterococcus faecalis ATCC 19433 (WDCM 00009))	< 100 colonies
Incubation: Half Fraser Broth: 24 ± 2 hours at 30 ± 1°C Confirmation on ALOA®-Medium: 44 ± 4 hours at 37 ± 1°C Confirmation on Tryptic Soy Agar: 24 ± 3 hours at 37 ± 1°C

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