MERCK 100093.0010 Fraser Lysterya Selectyve Supplement

MERCK 100093.0010 Fraser Lysterya Selectyve Supplement (SD.M.100093.0010)

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MERCK 100093.0010 Fraser Lysterya Selectyve Supplement

Description
Catalogue Number	100093
Replaces	1.00093.0017

Product Information
HS Code	3821 00 00
Quality Level	MQ200

Physicochemical Information
pH value	8.1 - 8.6 (H₂O, 20 °C) Aqueous solution

Safety Information according to GHS
Hazard Statement(s)	H318: Causes serious eye damage. H351: Suspected of causing cancer. H412: Harmful to aquatic life with long lasting effects.
Precautionary Statement(s)	P202: Do not handle until all safety precautions have been read and understood. P273: Avoid release to the environment. P280: Wear protective gloves/ protective clothing/ eye protection/ face protection/ hearing protection. P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P308 + P313: IF exposed or concerned: Get medical advice/ attention. P405: Store locked up.
Storage class	10 - 13 Other liquids and solids
WGK	WGK 3 highly hazardous to water
Disposal	3 Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.

Safety Information
Categories of danger	irritant, dangerous for the environment, toxic for reproduction

Storage and Shipping Information
Storage	Store at +2°C to +8°C.

Transport Information
Declaration (railroad and road) ADR, RID	Kein Gefahrgut
Declaration (transport by air) IATA-DGR	No Dangerous Good
Declaration (transport by sea) IMDG-Code	No Dangerous Good

Specifications
Growth promotion test according to ISO 11133 Mixed inoculum of Listeria, E.coli and E.faecalis Additionaly monoculture of E.coli and E.faecalis Pre-enrichment in Half Fraser Broth, afterwards enrichment in Fraser Broth Confirmation on ALOA®-Medium resp. Tryptic soy agar
Inoculum on reference medium - Listeria monocytogenes ATCC 19111 (WDCM 00020)	10 - 100
- Listeria monocytogenes ATCC 13932 (WDCM 00021)	10 - 100
- Escherichia coli ATCC 25922 (WDCM 00013)	≥ 1000
- Escherichia coli ATCC 8739 (WDCM 00012)	≥ 1000
- Enterococcus faecalis ATCC 19433 (WDCM 00009)	≥ 1000
- Enterococcus faecalis ATCC 29212 (WDCM 00087)	≥ 1000
Blacking - Listeria monocytogenes ATCC 19111 (WDCM 00020)	+
- Listeria monocytogenes ATCC 13932 (WDCM 00021)	+
- Escherichia coli ATCC 25922 (WDCM 00013)	-
- Escherichia coli ATCC 8739 (WDCM 00012)	-
- Enterococcus faecalis ATCC 19433 (WDCM 00009)	-
- Enterococcus faecalis ATCC 29212 (WDCM 00087)	-
Growth on ALOA® medium - Listeria monocytogenes ATCC 19111 (WDCM 00020)	> 10 CFU
- Listeria monocytogenes ATCC 13932 (WDCM 00021)	> 10 CFU
Colonies - Listeria monocytogenes ATCC 19111 (WDCM 00020)	bluegreen with non transparent zone
- Listeria monocytogenes ATCC 13932 (WDCM 00021)	bluegreen with non transparent zone
Growth on Tryptic soy agar - Escherichia coli ATCC 25922 (WDCM 00013)	no growth
- Escherichia coli ATCC 8739 (WDCM 00012)	no growth
- Enterococcus faecalis ATCC 29212 (WDCM 00087)	< 100 CFU
- Enterococcus faecalis ATCC 19433 (WDCM 00009)	< 100 CFU
Incubation: Half Fraser Broth: 24 hours at 30°C, aerobic Fraser Broth: 48 hours at 37°C, aerobic Confirmation on ALOA®-Medium: 48 hours at 37°C, aerobic Confirmation on Tryptic soy agar: 24 hours at 35°C, aerobic

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