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MERCK 109215.0500 Ziehl-Neelsen Carbol-Fuchsin Solution For Microscopy (SD.M.109215.0500)

Marka : MERCK
Fiyat : 1.881,96 ₺  + KDV
KDV Dahil : 2.258,35 ₺





 

 

MERCK 109215.0500 Ziehl-Neelsen Carbol-Fuchsin Solution For Microscopy

 

 

 
Description
Catalogue Number 109215
Synonyms Carbol fuchsin solution
Description Ziehl-Neelsen carbol-fuchsin solution
Overview Ziehl-Neelsen carbolfuchsin solution - for microscopy, is a ready-to use solution used in the Ziehl-Neelsen hot staining for the microscopic investigation of acid-fast bacteria(AFB). The cell wall of acid-fast bacteria has a high proportion of wax and lipids and hence absorbs dyes only very slowly. The most efficient staining method is the hot staining according to Ziehl-Neelsen. In this method, carbolfuchsin solution is applied to the specimen and heated. This heating process accelerates the rate at which the fuchsin dye is absorbed and thus also that of the formation of the mycolate-fuchsin complex in the cell wall. Once the acid-fast bacteria have absorbed the fuchsin dye, it is virtually impossible to decolorize them again, even when they are intensively treated with a decolorizing solution such as e. g. hydrochloric acid in ethanol (Cat. no. 100327). Accordingly, acid-fast bacteria are termed as acid- and alcohol-fast for staining, and are stained red in the microscopic visualization. Correspondingly, all non-acid-fast microorganisms are counterstained with an appropriate dye. In this instruction for use Löfföer's methylene blue solution(Cat. no. 101287) is used for counterstaining. Those three solutions are each 500 ml and are contained in the AFB-color modified staining kit (Cat. No.100497).
The 500 ml bottle of Ziehl-Neelsen carbolfuchsin solution provides approx. 250 stainings. Pretreatment of the specimens with Sputofluol® dissolves the bacteria from the surrounding viscid sputum and cell material. Ziehl-Neelsen carbolfuchsin solution - for microscopy is an IVD product and CE registered. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
Product Information
HS Code 3212 90 90
Quality Level MQ400
Applications
Application for microscopy
Physicochemical Information
Density 0.99 g/cm3 (20 °C)
Flash point 47 °C
Safety Information according to GHS
Hazard Statement(s) H226: Flammable liquid and vapour.
H314: Causes severe skin burns and eye damage.
H341: Suspected of causing genetic defects.
H410: Very toxic to aquatic life with long lasting effects.
Precautionary Statement(s) P210: Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
P273: Avoid release to the environment.
P280: Wear protective gloves/ protective clothing/ eye protection/ face protection/ hearing protection.
P303 + P361 + P353: IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water.
P304 + P340 + P310: IF INHALED: Remove person to fresh air and keep comfortable for breathing. Immediately call a POISON CENTER/doctor.
P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
Signal Word Danger
Storage class 3 Flammable liquids
WGK WGK 3 highly hazardous to water
Disposal 9
Carcinogenic compounds and flammable compounds labelled "Highly toxic" or "Toxic": container F. Alkyl sulfates are carcinogenic; Take particular care to avoid inhalation and skin contact. To neutralize alkyl sulfates, add dropwise (from a dropping funnel) to concentrated ice-cool Ammonia solution (Cat.No. 105426) with vigorous stirring. Before placing in container D, check the pH with pH Universal indicator strips (Cat.No. 109535).
Safety Information
Categories of danger flammable, harmful, corrosive, mutagenic
Storage and Shipping Information
Storage Store at +15°C to +25°C.
Transport Information
Declaration (railroad and road) ADR, RID UN 1992 , 3 (6.1), III
Declaration (transport by air) IATA-DGR UN 1992 , 3 (6.1), III
Declaration (transport by sea) IMDG-Code UN 1992 , 3 (6.1), III
Global Trade Item Number
Catalogue Number GTIN
1092150100 04022536112008
1092150500 04022536112015
1092150507 04054839116780
1092152500 04022536112039
Etiketler
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